EXTRACT OF GAMBIR BY PRODUCT AND FLORA ACTIVE CARBON AS ANTI BACTERIAL ACTIVITY TOWARD Bacillus subtilis

Abstract

A research was conducted with purpose to examine antibacterial activity (inhibition diameter zone ) on gambir extract and flora active carbon toward Bacillus subtilis. Research materials consisted of Bacillus subtilis, extract of Gambir by product and flora active carbon. The research was arranged in factorial ANOVA based on CRD that consisted of three replications and  four levels of A factors i.e. A1, A2, A3 and A4 with 0%, 10% , 20% and 30% (v/w) of extract of gambir by product respectively as well as four levels of B factors i.e. B1, B2, B3, and B4 with 0% , 0,1% , 0,2% and 0,3% active carbon respectively. if there were significant effects among variables it would be tested by Duncan’s multiple range test. Variable in the research was anti bacterial activity of the both factors toward Bacillus subtilis. The antibacterial activity of gambir by product as well as active carbon were also analyzed through comparing them to those of Kanamycin, Oxitetracycline sulfadiazine and peneciline with the antibacterial activity 0.6, 1.2, 0.0 and 0.0 cm respectively. With the highest value of significant or non significant antibacterial activity  gambir anti bacterial activity were 0.24, 0.35, 1.24 and 0.75 as well as that of active carbon were 0.15, 0.16, 0.90 and 0.51 respectively. The was a significant positive interaction between the both factors A and B.  Active carbon as single treatment had no effect on bacterial activity. It was concluded that combination of gambir extract and active carbon only effective in comparison to sulfadiazine as well as penecilin but had too low bacterial activity compared to that of kanamycin and oxitetracyclin.

Key words: B. subtilis, gambir by product extract, active carbon, bacterial activity.