The Dynamics of Protein Level and Protease Activity of Local Sheep Rumen Which Fed by Hydrophonic Maize Fodder and Other Forage in In-Vitro Method

  • Rifky Faradha Faculty of Animal Science, Jenderal Soedirman University, Purwokerto, Indonesia
  • Wardhana Suryapratama Faculty of Animal Science, Jenderal Soedirman University, Purwokerto, Indonesia
  • Sri Rahayu Faculty of Animal Science, Jenderal Soedirman University, Purwokerto, Indonesia
DOI: https://doi.org/10.20884/1.angon.2019.1.1.p21-27
Keywords: hydroponics, maize, fodder, forage, protein, enzym, protease

Abstract

Background. The aim of research was to examine the effect of hydroponic maize fodder and other forage towards Dynamics Protein Level and Enzym Protease Activity Digestive Rumen Sheep using In-Vitro Method. Materials and methods. The material that used in this research were three digestive rumen of
sheep. The research was held from January 14th, 2018 to March 7th, 2018 at the Laboratory of Science Feed and Nutrition, Faculty of Animal Science,Jenderal
Soedirman University, Purwokerto, Indonesia with in vitro method. The research was designed by Completed Randomized Design (CRD). The data was analyzed with analysis of variance (anova) and further testing by Honesty Significant Difference (HSD) test. The treatment consist of P1= 60 % Concentrate + 40 % Hydroponic Maize Fodder, P2 = 60 % Concentrate + 40 % Conventional Maize fodder, P3 = 60 % Concentrate + 40 % grass field and 6 repetition with 0 hours, 8 hours, 16 hours, 24 hours incubation. Data variable that measured are dynamics protein level and enzym protease activity. The result based on the test showed that provision of hydroponic maize fodder produce the highest dynamics protein level which is 0.0419 at the 8th hour of incubation compared to conventional maize fodder and field grasses. Results. Based on the results of the analysis of variance showed that the treatment of feeding various types of forages at a measurement of 0 hours of real incubation resulted (P<0.05) in the highest protease activity. Utilizing of hydroponic maize
fodder and maize fodder as a forage source produced the highest protease activity compared to field grass. The average of dynamics protein level based
on the treatment research P1, P2, dan P3from 8 hours, 16 hours, and 24 hours of incubation consecutively was 0.0206; 0.0079; 0.0026 percent per hours,
0.0056; 0.0109; 0.0139 percent per hours, 0.0020; 0.0031; 0.0080 percent per hours. The average of protease activity based on the treatment research P1, P2,
dan P3 from 0 hours, 8 hours, 16 hours, and 24 hours of incubation consecutively was 0.972; 1.107; 0.918 U/mg, 1.020; 0.965; 1.030 U/mg, 1.005; 0.947; 1.040 U/mg, 1.120; 0.978; 0.972 U/mg. Conclusion The highest protein dynamics level were seen in the hydroponic maize fodder treatment (P1) then followed by fodder maize treatment (P2) and field grass. Protease activity in fodder maize treatment (P2) showed the highest value followed by the treatment of hydroponic maize fodder (P1) then the lowest was the field grass treatment (P3).

References

Brock, F. M., C. W. Forsberg and J. G. Buchanan-Smith. 1982. Proteolytic activity of rumen microorganisms and effects of proteinase inhibitors. Appl. Environ.
microbiol. 44(3):561-569.
Carberry, C. A., D. A. Kenny, S. Han, M. S. McCabe, and S. M. Waters. 2012. Effect of phenotypic residual feed intake and dietary forage content on the rumen
microbial community of beef cattle. Appl. Environ. microbiol. 78(14): 4949-4958.
Forsbeg, C.W., L.K.A. Lovelock and J.G. Buchanan-smith. 1984. Protease Activities of Rumen. Appl. Environ. microbiol. 47(1): 101-110.
Khampa, S., M. Wanapat, C. Wachirapakorn, N. Nontaso, M.A. Wattiaux, and P. Rowlison. 2006. Effect of levels of sodium DL-malate supplementation on
ruminal fermentation efficiency of concentrates containing high levels of cassava chip in dairy steers. Aisan-Aust. J. Anim. Sci. 19: 368-375.
Larasati, T. P. 2018. Produk Fermentasi Rumen Sapi Potong Yang diberi Pakan Fodder Jagung Hidroponik Dan Hijauan Lain Secara In Vitro. Skripsi. Fakultas
Peternakan. Unsoed, Purwokerto
Oktafiani, D. R., U. H. Tanuwiria., dan R. Hidayat. 2015. Pengaruh Berbagai Umur Pemotongan Tanaman Rami (Boehmeria nivea) terhadap Produksi NH3 dan VFA
Cairan Rumen Domba (In Vitro). Students E-Journal. 4(3) : 1-13.
Raharjo, S., L.N. Nuswantara dan E.D. Purbajanti. 2017. Produksi dan Kandungan Nutrien Fodder Jagung Hidroponik dengan Media Perendaman dan Penggunaan
Dosis Pupuk yang Berbeda sebagai Pakan Alternatif Ruminansia. Tesis. Fakultas Peternakan dan Pertanian Universitas Dipenogoro, Semarang.
Soepranianondo, K. 2005. Dampak Isi Rumen Sapi sebagai Substitusi Rumput Raja terhadap Produk Metabolit pada Kambing Peranakan Etawa. Media Kedokteran
Hewan. 21 (2) : 94 – 96.
Suhardiyanto, H. 2011. Teknologi Hidroponik Untuk Budidaya Tanaman. IPB press, Bogor.
Sutardi, T. 1979. Ketahanan Protein Bahan Makanan Ternak terhadap Degradasi oleh Mikroba Rumen dan Manfaatnya bagi Peningkatan Produksi Ternak.
Prosiding Seminar dan Penunjang Peternakan. LPP, Bogor.
Published
2019-03-20
How to Cite
[1]
R. Faradha, W. Suryapratama, and S. Rahayu, “The Dynamics of Protein Level and Protease Activity of Local Sheep Rumen Which Fed by Hydrophonic Maize Fodder and Other Forage in In-Vitro Method”, ANGON: Journal of Animal Science and Technology, vol. 1, no. 1, pp. 21-27, Mar. 2019.
Issue
Vol 1 No 1 (2019): JURNAL ANGON
Section
Articles

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