THE EFFECTS OF DIFFERENT KINDS AND TIME OF STORAGE AT 5oC TEMPERATURE ON MOTILITY, VIABILITY, AND ABNORMALITY OF KAMPUNG ROOSTER SPERMATOZOA

Dipta Rilandana; Dadang Mulyadi Saleh; Aras Prasetiyo Nugroho

doi:10.20884/1.angon.2021.3.2.p184-191

Dipta Rilandana Animal Science Faculty, Jenderal Soedirman University
Dadang Mulyadi Saleh Animal Science Faculty, Jenderal Soedirman University
Aras Prasetiyo Nugroho Animal Science Faculty, Jenderal Soedirman University

DOI: https://doi.org/10.20884/1.angon.2021.3.2.p184-191

Keywords: kampung rooster, spermatozoa, motility, viability, abnormality

Abstract

Background. The aim of the study was to determine the interaction between types of diluents (Ringer's lactate, skimmed milk, and andromed) and storage time at 5°C on motility, viability, and abnormalities of kampung rooster spermatozoa. Materials and Methods. The research materials used were 7 kampung rooster aged 1-1.5 years as a spermatozoa producer, chicken feed, battery cage and its equipment, spermatozoa storage glass, a set of tools to test the quality of spermatozoa, and 1 ml syringe. Other materials are fresh spermatozoa from 7 free-range kampung rooster, skim milk, lactated Ringer, andromed, distilled water, microscopes, dropper pipettes, object glass, cover glass, cement box, thoma chamber, hematocrit pipette, 3% NaCl solution, eosin negrosin solution, refrigerator, and microtube. The research design used was a completely randomized design (CRD) with a factorial pattern of 2 factors: the P factor, namely the kind of diluent and the W factor, the time of storage at 5°C through 3 treatments. There were 3 replications in each treatment so that there were 27 experimental units with each treatment consisting of 3 different times. Results. The results showed that the interaction between different kinds of diluent treatments and storage time had a significant effect (P<0.05) on the viability of kampung rooster spermatozoa, while the interaction between treatments of types of diluents and storage time on motility and abnormalities of kampung rooster spermatozoa had no significant effect (P>0.05). ANOVA results had a very significant effect on the type of diluent (P<0.01) on motility, while the abnormal effect was not significant (P>0.05). Storage time had a significant (P<0.05) effect on abnormalities and had no significant (P>0.05) effect on motility. The results showed that the best motility of free-range kampung rooster spermatozoa was produced by Ringer's lactate diluent with storage for 10 minutes (92%). The best viability of kampung rooster spermatozoa was produced by andromed diluent with storage for 370 minutes (96.5%). Furthermore, the lowest abnormality of kampung rooster spermatozoa was produced by skim milk diluent with storage for 190 minutes (8.5%).

References

Agustian, F. M., Ihsan, N. M., & Isnaini, N. 2014. Pengaruh Lama Simpan Semen dengan Pengencer Tris Aminomethan Kuning Telur Pada Suhu Ruang Terhadap Kualitas Spermatozoa Kambing Boer. Jurnal Ternak Tropika. 15(2):1-6.
Anwar, Kholid. 2011. Panduan Sukses Beternak dan Bisnis Ayam Kampung. Yogyakarta: Penerbit Pinang Merah.
Arifiantini, R. I., Wresdiyati, T dan E. F. Retnani. 2006. Pengujian Morfologi Spermatozoa Sapi Bali (Bos sondaicus) Menggunakan Pewarnaan “Williams”. J. Indontrop. Anim. Agric. 31(2):105-110.
Danang, D. R., Isnaini, N., & Trisunuwati, P. 2012. Pengaruh Lama Simpan Semen Terhadap Kualitas Spermatozoa Ayam Kampung dalam Pengencer Ringer’s pada Suhu 4°C. Journal of Tropical Animal Production. 13(1):47-57.
Fauzan, M., M, Hartono, dan P.E. Santosa. 2009. Pengaruh Suhu dan Lama Thawing di Dataran Rendah terhadap Kualitas Semen Beku Sapi Brahman. Jurnal Ternak Tropika. 12(1):27-37.
Hafez, B. & Hafez, E.S.E. 2008. Reproduction in Farm Animals. 7th ed. Blackwell Publishing. Australia.
Herdis. 2012. Pengaruh Waktu Penampungan Semen Terhadap Gerakan Massa Spermatozoa dan Tingkah Laku Kopulasi Pejantan Domba Garut. J. Sains dan Teknologi Indonesia. 14:38-43.
Isnaeni, M., Faidiban, O. R., & Tethool, A. N. 2019. Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam). Jurnal Ilmu Peternakan dan Veteriner Tropis. Journal of Tropical Animal and Veterinary Science. 9(2):44-49.
Lubis TM. 2011. Motilitas Spermatozoa Ayam Kampung dalam Pengencer Air Kelapa, NaCl Fisiologis dan Air Kelapa-NaCl Fisiologis pada 25-29°C. Agripet. 11(2):45-50.
Malik, A. 2018. Potensi Bahan Pengencer Citrat dan Kuning Telur yang Berbeda Rasio terhadap Kualitas Spermatozoa Ayam Kampung. Potensi Bahan Pengencer Citrat dan Kuning Telur yang Berbeda Rasio terhadap Kualitas Spermatozoa Ayam Kampung. Ternak Tropika. 3(2): 54-58.
Nugroho, A. P., & Saleh, D. M. 2016. Motilitas dan Abnormalitas Spermatozoa Ayam Kampung dengan Pengencer Ringer Laktat-Putih Telur dan Lama Simpan pada Suhu 5°C Selama 48 Jam. Acta Veterinaria Indonesiana. 4(1):35-41.
Priyono, Sopiyana, S., & Kostaman, T. 2019. Kerja Finansial Manajemen Perkawinan Inseminasi Buatan dan Kawin Alam pada Ayam Lokal. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner, Bogor. 581-589.
Susilawati, T. 2011. Tingkat Keberhasilan Inseminasi Buatan dengan Kualitas dan Deposisi Semen yang Berbeda pada Sapi Peranakan Ongole. Journal of Tropical Animal Production. 12(2):15-24.
Varasofiari, L. N., Setiatin, E. T., & Sutopo, S. 2013. Evaluasi Kualitas Semen Segar Sapi Jawa Brebes berdasarkan Lama Waktu Penyimpanan. Animal Agriculture Journal. 2(1): 201-208.
Yu, I., & Leibo, S. P. 2002. Recovery of Motile, Membrane-intact Spermatozoa from Canine Epididymides Stored for 8 Days at 4°C. Theriogenology. 57(3): 1179-1190.
Yulnawati., Herdis., Maheswari, H., Boediono, A dan Rizal, M. 2009. Potensi Reproduksi dan Upaya Pengembangbiakan Kerbau Belang Tana Toraja. Seminar dan Lokakarya Nasional Kerbau.