CONCENTRATION OF VFA AND NH3 LIQUID OF SHEEP RUMENTS FLOURED WITH IN VITRO LEAVES AND PALM OIL

Afifah Dian Safitri; F M Suhartati; Efka Aris Rimbawanto

doi:10.20884/1.angon.2021.3.2.p149-155

Afifah Dian Safitri Animal Science Faculty, Jenderal Soedirman University
F M Suhartati Animal Science Faculty, Jenderal Soedirman University
Efka Aris Rimbawanto Animal Science Faculty, Jenderal Soedirman University

DOI: https://doi.org/10.20884/1.angon.2021.3.2.p149-155

Keywords: VFA, NH3, in vitro, lamb, moringa leaf flour, palm oil

Abstract

Background. The research entitled VFA and NH3 Concentrations of Rumen Fluid Given In Vitro Moringa oleifera Leaf Meal and Palm Oil, which was conducted on 27 June 2020 to 12 September 2020. Materials and Methods. The material used in the study was the rumen fluid of 3 sheep obtained shortly after slughter, and beforehand lamb have been fed with the same treatment as the study. The treatment rations used consisted of R₀ = control feed without moringa leaves and palm oil, R₁ = control feed + 2% moringa oleifera leaf meal from feed dry matter (DM), and R₂ = control feed + 2% moringa leaf meal from feed DM + 0.5% palm oil from feed DM. The method in the study was carried out in vitro according to Tilley and Terry (1963) which was modified by Sutardi (1979), the variable measured was the VFA concentration using the steam distillation method according to Krooman et al. (1967), while the rumen fluid NH₃ concentration was measured using the Conway (1940) microdifusion technique. Results. The results of the analysis of variance showed that moringa leaf meal and palm oil supplementation had no significant effect (P> 0.05) on the VFA and NH₃ concentrations of sheep rumen fluid in vitro, but the data obtained by supplementing Moringa leaf meal and palm oil could increase VFA 0,95% and NH₃ 0,94%. Conclusion. It was concluded that the use of 2% Moringa leaf powder supplementation and 0,5% palm oil increased the VFA concentration by 0,95% and NH₃ 0,94% of lamb rumen fluid in vitro.

nd NH₃ 0,94% of lamb rumen fluid in vitro.

References

Arum, I., S. Rahayu dan M. Bata. 2013. Pengaruh Pemberian Ekstrak Daun Waru (Hibiscus tiliaceus) pada Pakan Sapi Potong Lokal Terhadap Produksi VFA Total dan NH3 Secara In Vitro. Jurnal Ilmu Peternakan. 1(1): 21-38.
Conway, E.J. 1940. Micro-Diffusion Analysis and Volumetric Error. D. Van Nostrand Co., Inc. New York City, USA.
Dewi, O., N. N. Suryani, dan I.M. Mudita. 2020. Kecernaan bahan kering dan bahan organik secara in-vitro dari silase kombinasi batang pisang dengan kembang telang (Clitoria ternatea). Jurnal Peternakan Tropika. 8(1): 60-73.
Fathul, F. dan S. Wajizah. 2010. Penambahan mikromineral Mn dan Cu dalamransum terhadap aktivasi biofermentasi rumen domba secarain vitro. JITV. 15 (1) : 9-15.
Herdian, H., L. Istiqomah., A. Febrisiantosa., dan D. Setiabudi. 2011. Pengaruh Penambahan Daun Morinda Citrifolia sebagai Sumber Saponin terhadap Karakteristik Fermentasi, Defaunasi Protozoa, Produksi Gas dan Metana Cairan Rumen secara In Vitro. JITV. 16(2): 88-104.
Hikmawan, D., Erwanto, Muhtarudin, dan F. Fathul. 2019. Pengaruh Substitusi Rumput Laut (Eucheuma cottonii) dalam Pakan Rumput Gajah (Pennisetum purpureum) Terhadap Konsentrasi VFA Parsial Dan Estimasi Produksi Gas Metana Secara In-Vitro. Jurnal Riset dan Inovasi Peternakan. 3(1) : 12-18.
Krooman, R.P., J.H. Meyer, and W.J. Stielau, 1967. Steam distillation of Volatile Fatty Acid in Rumen Ingesta. Journal Dairy Sci. 50:73.
Marhaeniyanto, E., dan S. Susanti. 2014. Produk Fermentasi dan Produksi Gas secara In-Vitro dari Ransum yang Mengandung Daun Kelor (Moringa oleifera). Jurnal Buana Sains. 14(2) : 19-28.
Orskov, E. R. 1982. Protein Nutrition in Ruminant. Academic Press, New York.
Puastuti W., D. Yulistiani, dan I. W. Mathius. 2012. Respon Fermentasi Rumen dan Retensi Nitrogen dari Domba yang Diberi Protein Tahan Degradasi dalam Rumen. JITV. 17 (1) : 67-72.
Puastuti, W., D. Yulistiani, dan I.W.R Susana. 2014. Evaluasi Nilai Nutrisi Bungkil Inti Sawit yang Difermentasi dengan Kapang Sebagai Sumber Protein Ruminansia. JITV. 19(2) : 143-151.
Pudjihastuti, E., J. R. Bujung, dan C. L. Kaunang. 2019. Profil Karkas dan Status Hematologis Darah dari Sapi yang Diberi UGB. Jurnal MIPA UNSRAT. 8(3) : 168-171.
Sutardi, T. 1979. Ketahanan Protein Bahan Makanan Terhadap Degradasi oleh Mikroba Rumen dan Manfaatnya Bagi Peningkatan Produktifitas Ternak. Di dalam : Prosiding Seminar Penelitian dan Penunjang Peternakan. LPP IPB. Bogor.
Tilley, J.M. A. and R.A. Terry, 1963. The Relationship Between the Soluble Constitutent Herbage and Their Dry Matter Digestibility. Journal British Feed Science. 18: 104-111.
Uhi, H. T. 2006. Perbandingan Suplemen Katalitik dengan Bungkil Kedelai terhadap Penampilan Domba. Jurnal Ilmu Ternak. 6(1) : 1-6.
Widiyanti, R. A. 2015. Pemanfaatan Kelapa Menjadi VCO (Virgin Coconut Oil) sebagai Antibiotik Kesehatan dalam Upaya Mendukung. Prosiding Seminar Nasional Pendidikan Biologi. 577-584.
Yanuartono, A. Nururrozi, S. Indrajulianto, dan H. Purnamaningsih. 2019. Peran Protozoa pada Pencernaan Ruminansia dan Dampak Terhadap Lingkungan. Journal of Tropical Animal Production. 20(1) : 16-28.