EFEK DESINFEKTAN TERHADAP Leptospira interrogans serovar hardjo
Keywords:
desinfektan, Virkon S, sodium hipoklorit, L. interrogans serovar hardjo
Abstract
Leptospirosis merupakan penyakit zoonosis disebabkan oleh bakteri Leptospira patogen yang dapat menginfekasi hewan ternak, hewan liar, dan juga manusia. Hewan yang terinfeksi dapat menyebarkan bakteri ke lingkungan melalui urine dan menjadi sumber infeksi bagi hewan lain dan juga manusia sehingga desinfeksi kandang dan lingkungan penting dalam upaya pengendalian penyakit. Desinfektan yang sering digunakan dan mudah diperoleh yaitu Virkon S dan sodium hipoklorit. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian Virkon S dan sodium hipoklorit terhadap daya hidup bakteri Leptospira interrogans serovar hardjo. Kultur bakteri Leptospira interrogans serovar hardjo dengan konsentrasi 1,5 x 108 CFU/mL ditambahkan dengan larutan sodium hipoklorit dan Virkon S hingga menghasilkan campuran dengan konsentrasi 0,1%, 0,5%, dan 1% dan diinkubasi selama 5, 10, 15, 30, dan 45 menit. Pengamatan dilakukan dengan menggunakan mikroskop fase kontras. Hasil menunjukkan sodium hipoklorit dengan konsentrasi 1% paling cepat dapat mengeliminasi L. interrogans serovar hardjo pada menit ke-10 sedangkan Virkon S 0,1%, 0,5%, dan 1% belum dapat mengeliminasi bakteri pada pengamatan secara langsung hingga 45 menit. Sodium hipoklorit lebih efektif dibandingkan dengan Virkon S dalam mengeliminasi L. interrogans serovar hardjo. Oleh karena itu, peternak dianjurkan menggunakan sodium hipoklorit 1% untuk desinfeksi kandang dan lingkungan.
References
Alfanandyah, Z., SK Widyastuti, IH Utama, MP Profesi, dan D Hewan. 2016. Bentuk dan Kelainan Kuku Sapi Bali yang Dipelihara dalam Kandang Berlantai Keras. Indonesia Medicus Veterinus Januari. 5(1): 23–29.
Astudillo, VG, DW Hernández, JP Stadlin, LA Bernal, DAL Rodríguez. dan MA Hernández. 2012. Comparative seroprevalence of Leptospira interrogans in Colombian mammals along a climatic gradient. Journal of Zoo and Wildlife Medicine. 43(4) : 768–775.
Bharti, AR, JE Nally, JN Ricaldi, MA Matthias, MM Diaz, MA Lovett, PN Levett, RH Gilman, MR Willig, E Gotuzzo, dan JM Vinetz. 2003. Leptospirosis: a zoonotic disease of global importance. Lancet Infectious Diseases, 3 : 757–771.
Bierque, E., R Thibeaux, D Girault, ME Soupé-Gilbert, dan C Goarant. 2020. A systematic review of Leptospira in water and soil environments. PLoS ONE, 15(1)
Dunowska, M., PS Morley. dan DR Hyatt. 2005. The effect of Virkon®S fogging on survival of Salmonella enterica and Staphylococcus aureus on surfaces in a veterinary teaching hospital. Veterinary Microbiology, 105(3–4), 281–289.
Flores, B., K Escobar, JL Muzquiz, J Sheleby-Elías, B Mora, E Roque, D Torres, A Chávez, dan W Jirón. 2020. Detection of pathogenic leptospires in water and soil in areas endemic to leptospirosis in Nicaragua. Tropical Medicine and Infectious Disease. 5(3) : 1–10.
Fukuzaki, S. 2006. Mechanisms of Actions of Sodium Hypochlorite in Cleaning and Disinfection Processes. Biocontrol Science, 11(4) : 147–157.
Gabbert, LR, JD Smith, JG Neilan, GS Ferman, dan Mv Rasmussen. 2018. Smart Card Decontamination in a High-Containment Laboratory. Health Security. 16(4) : 244–251.
Gold, KK, PD Reed, DA Bemis, DL Miller, MJ Gray, dan JM Souza. 2013. Efficacy of common disinfectants and terbinafine in inactivating the growth of Batrachochytrium dendrobatidis in culture. Diseases of Aquatic Organisms. 107(1) : 77–81.
Haake, DA., dan WR Zuckert. 2014. The leptospiral outer membrane. Current Topics in Microbiology and Immunology. 387 : 187–221.
Hancox, L. R., le Bon, M., Dodd, C. E. R., & Mellits, K. H. (2013). Inclusion of detergent in a cleaning regime and effect on microbial load in livestock housing. Veterinary Record, 173(7), 167.
Hernandez, A., Martró, E., Matas, L., Martín, M., & Ausina, V. (2000). Assessment of in-vitro efficacy of 1% Virkon® against bacteria, fungi, viruses and spores by means of AFNOR guidelines. Journal of Hospital Infection, 46(3), 203–209.
Huang, C., S Tao, J Yuan, dan X Li. 2022. Effect of sodium hypochlorite on biofilm of Klebsiella pneumoniae with different drug resistance. American Journal of Infection Control. 50(8) : 922–928.
Jittimanee, J dan J Wongbutdee, J. 2019. Prevention and control of leptospirosis in people and surveillance of the pathogenic Leptospira in rats and in surface water found at villages. Journal of Infection and Public Health. 12(5) : 705–711.
Lilenbaum, W., R Varges, P Ristow, A Cortez, SO Souza, LJ Richtzenhain, dan SA Vasconcellos. 2009. Identification of Leptospira spp. carriers among seroreactive goats and sheep by polymerase chain reaction. Research in Veterinary Science. 87(1) : 16–19.
Maillard, JY. 2018. Resistance of Bacteria to Biocides. Microbiology Spectrum, 6(2).
McBride, AJA., DA Athanazio, MG Reis, dan AI Ko. 2005. Leptospirosis. Current Opinion in Infectious Diseases, 18 : 376–386.
Moslehifard, E., F Lotfipour, MR Anaraki, E Shafee, S Tamjid-Shabestari, T Ghaffari, dan E Moslehifard. 2015. Efficacy of Disinfection of Dental Stone Casts: Virkon Versus Sodium Hypochlorite. Journal of Dentistry, Tehran University of Medical Sciences. 12(3) : 206–215.
Mulyani, GT, B Sumiarto, dan Yuriati. 2014. Pembelian Ternak dan Kelembaban Tinggi Merupakan Faktor Risiko Leptospirosis pada Sapi di Girimulyo, Kulon Progo, Jogjakarta. Jurnal Veteriner, 15(2) : 199–204.
Nursanni, B., DM Yulanto, dan S Rahmadani. 2022. Pembinaan Desinfeksi Kandang pada Peternakan Rakyat Sebagai Upaya Pencegahan Wabah Penyakit Mulut dan Kuku. SAFARI :Jurnal Pengabdian Masyarakat Indonesia. 2(4) : 101–108.
Picardeau, M., DM Bulach, C Bouchier, RL Zuerner, N Zidane, PJ Wilson, S Creno, ES Kuczek, S Bommezzadri, JC Davis, A McGrath, MJ Johnson, C Boursaux-Eude, T Seemann, Z Rouy, RL Coppel, JI Rood, A Lajus, JK Davies, B Adler. 2008. Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis. PLoS ONE. 3(2).
Putra, F. AIAN Hidayat, dan T Afirianto. 2018. Penentuan Kelayakan Kandang Sapi Menggunakan Analytic Hierarcy Process-Weighted (AHP-WP) [Studi Kasus UPT Pembibitan Ternak Dan Hijauan Makanan Ternak Singosari]. Jurnal Pengembangan Teknologi Informasi Dan Ilmu Komputer. 2(10) : 2548–2964.
Saha, O., Rakhi, N. N., Istiaq, A., Islam, I., Sultana, M., Hossain, M. A., & Rahaman, M. M. (2020). Evaluation of Commercial Disinfectants against Staphylococcus lentus and Micrococcus spp. Of Poultry Origin. Veterinary Medicine International, 2020.
Sunaryo, S., & Priyanto, D. (2022). Leptospirosis in rats and livestock in Bantul and Gunungkidul district, Yogyakarta, Indonesia. Veterinary World, 15(6), 1449–1455.
Tappero JW, Ashford DA, Perkins BA. Leptospira species (leptospirosis). In: Mandell GL, Bennett JE, Dolin R, editors. Principles and practice of infectious diseases, Vol.2, 5th ed. Philadelphia: Churchill Livingstone; 2000. p.2495–501
Vincent, A. T., Schiettekatte, O., Goarant, C., Neela, V. K., Bernet, E., Thibeaux, R., Ismail, N., Khalid, M. K. N. M., Amran, F., Masuzawa, T., Nakao, R., Korba, A. A., Bourhy, P., Veyrier, F. J., & Picardeau, M. 2019. Revisiting the taxonomy and evolution of pathogenicity of the genus Leptospira through the prism of genomics. PLoS Neglected Tropical Diseases, 13(5).
Warnasekara, J., Srimantha, S., Senavirathna, I., Kappagoda, C., Farika, N., Nawinna, A., & Agampodi, S. 2022. The variable presence of Leptospira in the environment; an epidemiological explanation based on serial analysis of water samples. PLoS ONE, 17(2).
Widiasih, D. A., Lindahl, J. F., Artama, W. T., Sutomo, A. H., Kutanegara, P. M., Mulyani, G. T., Widodo, E., Djohan, T. S., & Unger, F. 2021. Leptospirosis in ruminants in yogyakarta, indonesia: A serological survey with mixed methods to identify risk factors. Tropical Medicine and Infectious Disease, 6(2).
Xu, Y., Zhu, Y., Wang, Y., Chang, Y. F., Zhang, Y., Jiang, X., Zhuang, X., Zhu, Y., Zhang, J., Zeng, L., Yang, M., Li, S., Wang, S., Ye, Q., Xin, X., Zhao, G., Zheng, H., Guo, X., & Wang, J. (2016). Whole genome sequencing revealed host adaptation-focused genomic plasticity of pathogenic Leptospira. Scientific Reports, 6.
Astudillo, VG, DW Hernández, JP Stadlin, LA Bernal, DAL Rodríguez. dan MA Hernández. 2012. Comparative seroprevalence of Leptospira interrogans in Colombian mammals along a climatic gradient. Journal of Zoo and Wildlife Medicine. 43(4) : 768–775.
Bharti, AR, JE Nally, JN Ricaldi, MA Matthias, MM Diaz, MA Lovett, PN Levett, RH Gilman, MR Willig, E Gotuzzo, dan JM Vinetz. 2003. Leptospirosis: a zoonotic disease of global importance. Lancet Infectious Diseases, 3 : 757–771.
Bierque, E., R Thibeaux, D Girault, ME Soupé-Gilbert, dan C Goarant. 2020. A systematic review of Leptospira in water and soil environments. PLoS ONE, 15(1)
Dunowska, M., PS Morley. dan DR Hyatt. 2005. The effect of Virkon®S fogging on survival of Salmonella enterica and Staphylococcus aureus on surfaces in a veterinary teaching hospital. Veterinary Microbiology, 105(3–4), 281–289.
Flores, B., K Escobar, JL Muzquiz, J Sheleby-Elías, B Mora, E Roque, D Torres, A Chávez, dan W Jirón. 2020. Detection of pathogenic leptospires in water and soil in areas endemic to leptospirosis in Nicaragua. Tropical Medicine and Infectious Disease. 5(3) : 1–10.
Fukuzaki, S. 2006. Mechanisms of Actions of Sodium Hypochlorite in Cleaning and Disinfection Processes. Biocontrol Science, 11(4) : 147–157.
Gabbert, LR, JD Smith, JG Neilan, GS Ferman, dan Mv Rasmussen. 2018. Smart Card Decontamination in a High-Containment Laboratory. Health Security. 16(4) : 244–251.
Gold, KK, PD Reed, DA Bemis, DL Miller, MJ Gray, dan JM Souza. 2013. Efficacy of common disinfectants and terbinafine in inactivating the growth of Batrachochytrium dendrobatidis in culture. Diseases of Aquatic Organisms. 107(1) : 77–81.
Haake, DA., dan WR Zuckert. 2014. The leptospiral outer membrane. Current Topics in Microbiology and Immunology. 387 : 187–221.
Hancox, L. R., le Bon, M., Dodd, C. E. R., & Mellits, K. H. (2013). Inclusion of detergent in a cleaning regime and effect on microbial load in livestock housing. Veterinary Record, 173(7), 167.
Hernandez, A., Martró, E., Matas, L., Martín, M., & Ausina, V. (2000). Assessment of in-vitro efficacy of 1% Virkon® against bacteria, fungi, viruses and spores by means of AFNOR guidelines. Journal of Hospital Infection, 46(3), 203–209.
Huang, C., S Tao, J Yuan, dan X Li. 2022. Effect of sodium hypochlorite on biofilm of Klebsiella pneumoniae with different drug resistance. American Journal of Infection Control. 50(8) : 922–928.
Jittimanee, J dan J Wongbutdee, J. 2019. Prevention and control of leptospirosis in people and surveillance of the pathogenic Leptospira in rats and in surface water found at villages. Journal of Infection and Public Health. 12(5) : 705–711.
Lilenbaum, W., R Varges, P Ristow, A Cortez, SO Souza, LJ Richtzenhain, dan SA Vasconcellos. 2009. Identification of Leptospira spp. carriers among seroreactive goats and sheep by polymerase chain reaction. Research in Veterinary Science. 87(1) : 16–19.
Maillard, JY. 2018. Resistance of Bacteria to Biocides. Microbiology Spectrum, 6(2).
McBride, AJA., DA Athanazio, MG Reis, dan AI Ko. 2005. Leptospirosis. Current Opinion in Infectious Diseases, 18 : 376–386.
Moslehifard, E., F Lotfipour, MR Anaraki, E Shafee, S Tamjid-Shabestari, T Ghaffari, dan E Moslehifard. 2015. Efficacy of Disinfection of Dental Stone Casts: Virkon Versus Sodium Hypochlorite. Journal of Dentistry, Tehran University of Medical Sciences. 12(3) : 206–215.
Mulyani, GT, B Sumiarto, dan Yuriati. 2014. Pembelian Ternak dan Kelembaban Tinggi Merupakan Faktor Risiko Leptospirosis pada Sapi di Girimulyo, Kulon Progo, Jogjakarta. Jurnal Veteriner, 15(2) : 199–204.
Nursanni, B., DM Yulanto, dan S Rahmadani. 2022. Pembinaan Desinfeksi Kandang pada Peternakan Rakyat Sebagai Upaya Pencegahan Wabah Penyakit Mulut dan Kuku. SAFARI :Jurnal Pengabdian Masyarakat Indonesia. 2(4) : 101–108.
Picardeau, M., DM Bulach, C Bouchier, RL Zuerner, N Zidane, PJ Wilson, S Creno, ES Kuczek, S Bommezzadri, JC Davis, A McGrath, MJ Johnson, C Boursaux-Eude, T Seemann, Z Rouy, RL Coppel, JI Rood, A Lajus, JK Davies, B Adler. 2008. Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis. PLoS ONE. 3(2).
Putra, F. AIAN Hidayat, dan T Afirianto. 2018. Penentuan Kelayakan Kandang Sapi Menggunakan Analytic Hierarcy Process-Weighted (AHP-WP) [Studi Kasus UPT Pembibitan Ternak Dan Hijauan Makanan Ternak Singosari]. Jurnal Pengembangan Teknologi Informasi Dan Ilmu Komputer. 2(10) : 2548–2964.
Saha, O., Rakhi, N. N., Istiaq, A., Islam, I., Sultana, M., Hossain, M. A., & Rahaman, M. M. (2020). Evaluation of Commercial Disinfectants against Staphylococcus lentus and Micrococcus spp. Of Poultry Origin. Veterinary Medicine International, 2020.
Sunaryo, S., & Priyanto, D. (2022). Leptospirosis in rats and livestock in Bantul and Gunungkidul district, Yogyakarta, Indonesia. Veterinary World, 15(6), 1449–1455.
Tappero JW, Ashford DA, Perkins BA. Leptospira species (leptospirosis). In: Mandell GL, Bennett JE, Dolin R, editors. Principles and practice of infectious diseases, Vol.2, 5th ed. Philadelphia: Churchill Livingstone; 2000. p.2495–501
Vincent, A. T., Schiettekatte, O., Goarant, C., Neela, V. K., Bernet, E., Thibeaux, R., Ismail, N., Khalid, M. K. N. M., Amran, F., Masuzawa, T., Nakao, R., Korba, A. A., Bourhy, P., Veyrier, F. J., & Picardeau, M. 2019. Revisiting the taxonomy and evolution of pathogenicity of the genus Leptospira through the prism of genomics. PLoS Neglected Tropical Diseases, 13(5).
Warnasekara, J., Srimantha, S., Senavirathna, I., Kappagoda, C., Farika, N., Nawinna, A., & Agampodi, S. 2022. The variable presence of Leptospira in the environment; an epidemiological explanation based on serial analysis of water samples. PLoS ONE, 17(2).
Widiasih, D. A., Lindahl, J. F., Artama, W. T., Sutomo, A. H., Kutanegara, P. M., Mulyani, G. T., Widodo, E., Djohan, T. S., & Unger, F. 2021. Leptospirosis in ruminants in yogyakarta, indonesia: A serological survey with mixed methods to identify risk factors. Tropical Medicine and Infectious Disease, 6(2).
Xu, Y., Zhu, Y., Wang, Y., Chang, Y. F., Zhang, Y., Jiang, X., Zhuang, X., Zhu, Y., Zhang, J., Zeng, L., Yang, M., Li, S., Wang, S., Ye, Q., Xin, X., Zhao, G., Zheng, H., Guo, X., & Wang, J. (2016). Whole genome sequencing revealed host adaptation-focused genomic plasticity of pathogenic Leptospira. Scientific Reports, 6.
Published
2023-07-23
How to Cite
Putri, R., Susanti, S., & Mulyadi, A. (2023). EFEK DESINFEKTAN TERHADAP Leptospira interrogans serovar hardjo. PROSIDING SEMINAR NASIONAL TEKNOLOGI AGRIBISNIS PETERNAKAN (STAP), 10, 322-327. Retrieved from http://jnp.fapet.unsoed.ac.id/index.php/psv/article/view/2275
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