KAJIAN METODE TITRASI FORMOL DALAM PENGUKURAN PROTEIN SUSU SEGAR MELALUI VALIDASI METODE ANALISIS (VMA)

Hermawan Setyo Widodo; Jodi Susanto; Yusuf Subagyo; Afduha Nurus Syamsi; Merryafinola Ifani

doi:10.20884/1.angon.2022.4.3.p303-309

Hermawan Setyo Widodo Fakultas Peternakan, Universitas Jenderal Soedirman, Purwokerto
Jodi Susanto Fakultas Peternakan, Universitas Jenderal Soedirman, Purwokerto
Yusuf Subagyo Fakultas Peternakan, Universitas Jenderal Soedirman, Purwokerto
Afduha Nurus Syamsi Fakultas Peternakan, Universitas Jenderal Soedirman, Purwokerto
Merryafinola Ifani Fakultas Peternakan, Universitas Jenderal Soedirman, Purwokerto

DOI: https://doi.org/10.20884/1.angon.2022.4.3.p303-309

Abstract

Background. This research was conducted to determine the feasibility of the formol titration method in measuring protein content in fresh milk. Material and method. The material used in this study was a sample of fresh cow's milk. Standard milk was prepared by adding hydrolyzed casein to obtain protein content of 10%, 8%, 6%, 4% and 2%. Each standard was triplicate tested to obtain LOD, LOQ, accuracy and precision calculations. The data obtained is then presented descriptively. Results. The results obtained show that the formol titration method achieves an LOD value of 2.12%, LOQ of 6.41%, an accuracy rate of 145.56% and a precision of 2;4;6;8;10% are 3.58;4.40;3.12;3.58;2.58%. The resulting linear curve has a regression equation Y=2.0228X – 2.785 with an R2 value of 0.957. Conclusion. It can be concluded that the measurement of milk protein using the formol titration method is quite good, but less accurate because it tends to be overquantify.

References

Anindita, N.S. dan D.S. Soyi. 2017. Studi Kasus: Pengawasan Kualitas Pangan Hewani melalui Pengujian Kualitas Susu Sapi yang Beredar di Kota Yogyakarta Case Study: Animal Food Quality Control Through Moving Cow Milk Quality Testing In Yogyakarta. Jurnal Peternakan Indonesia, 19(2):96–105.
Fatimah, S.F., V. Aisyah dan L.H. Nurani. 2018. Validasi metode analisis b-karoten dalam ekstrak etanol 96% Spirulina maxima dengan spektrofotometri visibel. Media Farmasi, 15(1):1-13.
Harmita. 2004. Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya. Majalah Ilmu Kefarmasian, 1(3):117- 135.
Lawson, L. 1996. Evaluation of Calibration Curve Linearity. Guidance Memo. No. 96-007 pp:1-9.
Ling, E. R. 1963. A text Book of Dairy Chemistry Vol. 2. 3rd Ed., Chapman and Hall, London UK.
Miarsono, S., W.R. Putri, dan J.W.A.P. 2021. Perbandingan Kadar Lemak, Protein dan Bahan Kering Tanpa Lemak (BKTL) pada Susu Sapi Segar di Kota Kediri dan Kabupaten Kediri. Jurnal Ilmiah Fillia Cendekia, 6(1):33–35.
Oka, B., M. Wijaya dan K. Kadirman. 2018. Karakterisasi Kimia Susu Sapi Perah di Kabupaten Sinjai. Jurnal Pendidikan Teknologi Pertanian, 3(2):195.
Pyne, G.T. 1932.The formol titration of milk. Biochem. J, 26:1005, 27: 915.
Standar Nasional Indonesia (SNI). 2011. SNI No.3141.1:2011 mengenai susu segar. Badan Standarisasi Nasional, Jakarta.
Utami, K.B., L.E. Radiati dan P. Surjowardojo. 2018. Kajian Kualitas Susu Sapi Perah PFH (Studi Kasus pada Anggota Kope-Rasi Agro Niaga di Kecamatan Jabung Kabupaten Malang). Jurnal Ilmu-Ilmu Peternakan, 24(2):58–66.
Widodo, H.S., T.Y. Astuti, P. Soediarto dan A.N. Syamsi. 2021. Identification of Goats’ and Cows’ Milk Protein Profile in Banyumas Regency by Sodium Dedocyl Sulphate Gel Electrophoresis (Sds-Page). Animal Production, 23(32):27–33.
Widodo, H.S., T.W. Murti, A. Agus dan W. Widodo. 2019. Mengidentifikasi Peptida Bioaktif Angiotensin Converting Enzyme-Inhibitor (Acei) dari Kasein Β Susu Kambing dengan Polimorfismenya melalui Teknik In Silico. Jurnal Aplikasi Teknologi Pangan, 7(4):180–185.

STUDY OF FORMOL TITRATION METHOD ON PROTEIN QUANTIFICATION OF FRESH MILK BY ANALITICAL METHOD VALIDATION

Abstract

References